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Info.
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Vol.2 - No.1 (2008.03.20) |
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Title
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Microelectrode Array for Electrochemiluminescence-based DNA Detection |
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Authors
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Sukdeb Pal1, Min Jung Kim1& Joon Myong Song1 |
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Institutions
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1Research Institute of Pharmaceutical Sciences and College of Pharmacy, Seoul National University, Seoul 151-742, Korea Correspondence and requests for materials should be addressed to J.M. Song (jmsong@snu.ac.kr) |
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Abstract
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This paper reports on the design, fabrication and testing of microelectrode arrays for electrochemiluminescence-based detection and quantitative analysis of DNA. To maximize the anode surface area in a small real estate different geometrical configurations of an anode such as wave-shaped, comb-shaped, and straight parallel coplanar arrangements with suitably designed cathode were designed. An asymmetric Ru-ligand complexe Ru(bpy)2(dcbpy)(PF6)2[bpy, 2,2??bipyridine; dcbpy, 4,4??dicarboxyl-2,2??bipyridine] was used as an electro-chemiluminescing DNA label. The detection and quantification of singlestranded DNA using the developed microelectrode arrays are presented. Preliminary tests yielded a etection limit of 10 nmol of Ru (II) chelate labeled DNA with a sample volume as low as 1 關L. The microelectrode array system can be integrated with a bipolar semiconductor photodiode array chip to fabricate a highly compact embodiment for on-chip handling of solutions and electrochemiluminescence detection.
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Keyword
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Electrochemiluminescence, Microelectrode, DNA Introduction |
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PDF File
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