| Info. | Vol.2 - No.2 (2008.06.20) |
|---|---|
| Title | Electrochemical Analysis of Glycated Hemoglobin Based on the Biospecificity and Electron-Transferring Capability of Ferroceneboronic Acid |
| Authors | Seo Young Son1 & Hyun C. Yoon1 |
| Institutions | 1Department of Molecular Science & Technology, Ajou University, Suwon 443-749, Korea Correspondence and requests for materials should be addressed to H.C. Yoon (hcyoon@ajou.ac.kr) |
| Abstract | We have developed a biosensing strategy for glycated proteins in red blood cells. Glycated hemoglobin (HbA1C) is recognized as an important target molecule to improve diabetes control and treatment. We employed ferroceneboronic acid (FcBA) as the signaling molecule, having both a signaling capability and biospecificity for target proteins. The boronic acid functional group in FcBA has an ability to form a covalent bond between its diol group and the cisdiol group from the carbohydrate chain on the surface of HbA1C. The FcBA was reacted with samples containing HbA1C, and the resulting conjugate was purified by a CENTRICON짰 membrane. After the solution-phase purification and concentration adjustment step, the electrochemical signal was registered. From the cyclic voltammetry, the detection range obtained for HbA1C was around 0.1-1,000 關g/mL. By employing bioelectrocatalytic signal amplification on the glucose oxidase (GOX)-modified electrode, we were able to enhance the biosensor sensitivity. This study can be implemented in a diagnostic biosensor and is applicable to other glycoproteins having analytical needs. |
| Keyword | Glycated hemoglobin, HbA1C, Ferroceneboronic acid, Electrochemical biosensor |
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