|
Info.
|
Vol.2 - No.1 (2008.03.20) |
|
Title
|
An Integrated Nanoporous Chip for Detecting Single DNA Molecules |
|
Authors
|
Rafael Mulero1 & Min Jun Kim1
|
|
Institutions
|
1Department of Mechanical Engineering & Mechanics, Drexel
University, Philadelphia, PA 19104, U.S.A
|
|
Abstract
|
We have developed a novel approach for ultra-fast DNA analysis by measurement of a parallel optical readout of DNA translocation through solid-state nanopore arrays. Parallelism is achieved through the fabrication of solid-state arrays of single-nanometer resolution pores and the simultaneous optical readout of DNA translocation utilizing scanning confocal microscopy. The optical readout of the arrays circumvents the previously necessary direct electrical addressing of each pore, reducing the method’s complexity. We present new nanofabrication techniques to create optipore arrays in 50 nm thick silicon nitride membranes using scanning transmission electron microscopy (STEM) and discuss our progress toward an ultra-fast high throughput DNA analysis.
|
|
Keyword
|
DNA, Ionic current blockade, Optical readout, Optipore array, STEM
|
|
PDF File
|
|
