| Info. | Vol.15 - No.1 (2021.03.20) |
|---|---|
| Title | Assembly and Disassembly of the Micropatterned Collagen Sheets Containing Cells for Location‑Based Cellular Function Analysis |
| Authors | Jaejung Son1, Hee‑Hoon Kim2, Jun‑Hee Lee2, Won‑Il Jeong2 and Je‑Kyun Park1,3(jekyun@kaist.ac.kr) |
| Institutions | 1Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak‑ro, Yuseong‑gu, Daejeon 34141, Republic of Korea 2Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak‑ro, Yuseong‑gu, Daejeon 34141, Republic of Korea 3KAIST Institute for Health Science and Technology, 291 Daehak‑ro, Yuseong‑gu, Daejeon 34141, Republic of Korea |
| Abstract | The liver has complex microenvironments, where parenchymal hepatocytes and non-parenchymal cells coexist. Hepatocytes exhibit different metabolic functions depending on their location by the oxygen gradient and the transcriptional changes of genes, which is called liver zonation. Three-dimensional (3D) liver tissue engineering has reproduced the complex microenvironments, but there is a limitation in analyzing them by location. In this study, a novel 3D tissue-level hepatic cell culture platform is developed via stacking the manipulable collagen sheets to spatially analyze the reconstructed metabolic zonation. The controlled assembly of the sheets containing hepatocytes and endothelial cells, respectively, creates a 3D co-culture environment that improves hepatic function. In addition, the sheet micropatterning can be used to control the accessibility of oxygen and nutrients in the stacked sheets. The disassembly of the stacked sheets enables a layer-by-layer analysis and allows us to confirm the metabolic zonation qualitatively. A demonstration of acetaminophen-induced liver injury using the stacked sheets shows the improved drug sensitivity by co-culture and chemical induction and presents the quantitative results of the different cellular responses to the drug by layers according to metabolic zonation. Therefore, this platform is expected to be used for an in-depth analysis of drug toxicity in complex tissues via spatial analysis. |
| Keyword | Collagen · Cytotoxicity · Hydrogel sheet · Metabolic zonation · Modular tissue engineering |
| PDF File |
 # 2010년도 발행분 부터는 Springer 의 BioChip Journal 페이지에서 전문을 열람하실 수 있습니다.
# 학회회원 로그인 후 [ Springer BioChip Journal 열람하기 ] 버튼을 클릭하시면 새창으로 열립니다. |
