| Info. | Vol.16 - No.3 (2022.09.20) |
|---|---|
| Title | Enzymatic Reaction Automation in Nanodroplet Microfluidic for the Quality Control of Monoclonal Antibodies |
| Authors | Y. Ladner1,*, D. Liu1, J. Montels1, J. Morel2, & C. Perrin1
*Y. Ladner yoann.ladner@umontpellier.fr |
| Institutions | 1Institut des Biomolécules Max Mousseron (IBMM), UMR 5247-CNRS-UM-ENSCM, Université de Montpellier, Montpellier, France
2Département de Rhumatologie, Université de Montpellier, Hôpital Lapeyronie, 34295 Montpellier cedex 5, France |
| Abstract | The present work is a proof-of-concept study that nanodroplet microfluidics technology is a tool for the quality control of monoclonal antibodies (mAb). The aqueous nanodroplets mixing on-demand inside the microfluidic channel permits to perform enzymatic reactions in an automated and controlled manner. First of all, nanodroplets generation and mixing were optimized regarding the choice of organic solvent and pressures applied on inlet ports. This optimization phase permits to perfectly control the amount of each reactant mixed in each nanodroplet. Then, in-line degradation of fluorescein isocyanate (FITC) in presence of the Horseradish peroxidase (HRP) enzyme was realized in a microchannel to show the ability of nanodroplet microfluidics technology to generate biochemical reactions. Enzymatic reactions involving trypsin and a monoclonal antibody (trastuzumab) (bottom-up approach) were also realized at a different enzyme to substrate (E/S) ratio by controlling the nanodroplets volume mixed inside microchannels. The determination of the amount of HRP grafted on the active binding sites of mAb surfaces (labeled molecule used in analytical biochemistry assay) was also realized. Successful and repeatable reactions were obtained demonstrating the power of the proposed methodology. |
| Keyword | Droplet microfluidics · Enzymatic nanoreactor · In-line digestion · Monoclonal antibodies (mAb) |
| PDF File |
 # 2010년도 발행분 부터는 Springer 의 BioChip Journal 페이지에서 전문을 열람하실 수 있습니다.
# 학회회원 로그인 후 [ Springer BioChip Journal 열람하기 ] 버튼을 클릭하시면 새창으로 열립니다. |
