| Info. | Vol.17 - No.3 (2023.09.20) |
|---|---|
| Title | Fabrication of Hydrogel Microchannels Using Aqueous Two-Phase Printing for 3D Blood Brain Barrier |
| Authors | Hyunjik Oh 1,5 , Minjin Kang 4 , Eunji Bae 3,5 , Yonghun Jung 2 , Jinhui Cho 5 , Joscillyn Poirier 8 , Jong Sung Kim 9 , John P. Frampton 6,7*, Nakwon Choi 4*, Seok Chung 2*
Hyunjik Oh: First author. *John P. Frampton john.frampton@dal.ca *Nakwon Choi nakwon.choi@kist.re.kr *Seok Chung sidchung@korea.ac.kr |
| Institutions | 1Department of Bio-Convergence Engineering, College of Health Science, Korea University, Seoul,Korea
2School of Mechanical Engineering, Korea University, Seoul, South Korea 3Department of Biomicrosystem Technology, Korea University, Seoul, South Korea 4 Center for Brain Technology Brain Science Institute, Korea Institute of Science and Technology, Seoul, South Korea 5MicroFIT Research and Business Development Institute, Gyeonggi-do, South Korea 6School of Biomedical Engineering, Dalhousie University, Halifax, Nova Scotia, Canada 7Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia, Canada 8Department of Process Engineering and Applied Science, Dalhousie University, Halifax, Nova Scotia, Canada 9 Department of Occupational and Environmental Health, College of Public Health, University of Iowa, Iowa City, IA 52242, USA |
| Abstract | The blood-brain barrier (BBB) surrounds brain cells and prevents external substances from entering the brain through blood vessels. This complicates drug delivery to brain cells, but drugs that can cross the BBB have been developed recently, expanding the scope of treatment for brain diseases. However, traditional biological research typically relies on simple monolayer cell cultures that do not reflect the complex functional properties of human tissues and organs or their responses to external stimuli. Bioprinting technology is gradually overcoming the drawbacks of in vitro models by applying techniques, such as simulating 3D structures, which cannot be realized by biological models, utilizing biocompatible materials and mass cell culture at the tissue level; however, it has been limited to printing microstructural patterns. The in vitro model presented here printed the BBB microstructure in a liquid state, eliminating many defects inherent to printing on a fl at surface in air. The aqueous two-phase printing (ATPP) material consisted of a composite matrix capable of phase separation, where three different cell types could be cultured to create a BBB model. The ATPP model will help in central nervous system disease research, drug screening, and drug discovery, because it provides an environment where the nutrient supply and drug concentration of cells can be controlled. |
| Keyword | Blood–brain barrier, Bioprinting, Aqueous two-phase printing, 3D microstructure, Drug screening |
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