| Info. | Vol.3 - No.1 (2009.03.20) |
|---|---|
| Title | The Mach-Zehnder Interferometer Based on Silicon Oxides for Label Free Detection of C-reactive Protein (CRP) |
| Authors | Jongin Hong1, Daesung Yoon2 & Tae Song Kim3 |
| Institutions | 1Department of Chemistry, Imperial College London,
South Kensington Campus, London SW7 2AZ, UK 2Department of Biomedical Engineering, Yonsei University, 234 Maeji-ri, Heungup-myeon, Wonju-si, Gangwon-do 220-710, Korea 3Korea Institute of Science and Technology, 39-1 Hawolgok-dong, Seongbuk-gu, Seoul 136-791, Korea Correspondence and requests for materials should be addressed to J. Hong (hong.jongin@gmail.com) |
| Abstract | The C-reactive protein (CRP) is a very important inflammation biomarker and a major predictor of cardiovascular disease (CVD) in human. We demonstrated here on a new approach to determine the binding of label free CRP to monoclonal anti-CRP using the Mach-Zehnder interferometer based on silicon oxides with the detection limit of 1 ng/mL or even less at the operating wavelength of 1,550 nm. The building block, single-mode waveguide of total internal reflection, was precisely designed using a finite element method (FEM) and so we suggested the guideline of microfabrication to achieve it. We also firstly introduced the calixcrown self-assembled monolayers (SAMs), which guaranteed the activity of capture proteins and the correct orientation, to immobilize the monoclonal anti-CRP on PECVD silicon oxides into the biosensors based on the interaction of the evanescent field of the guided light with the surrounding environment. |
| Keyword | C-reactive protein, Mach-Zehnder interferometer, Biosensor, Finite element method, Silicon oxide |
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