| Info. | Vol.3 - No.1 (2009.03.20) |
|---|---|
| Title | Comparison of Two In vivo Models for Evaluation of B52 Function |
| Authors | Misun Jung1,3, Eunkyung Kim1, Dong-ki Lee2& Soyoun Kim1 |
| Institutions | 1Department of Biomedical Engineering, Dongguk University,
Seoul 100-715 Korea 2Department of Chemistry and BK21 School of Chemical Materials Science, Sungkyunkwan University, Suwon 440-746 Korea 3PCL Inc., Research Complex 2, Sungkyunkwan University, Suwon 440-746 Korea Correspondence and requests for materials should be addressed to S. Kim (skim@dongguk.edu, skim99@paran.com) and D.K. Lee (dklee@skku.edu) |
| Abstract | B52/SRp55 is a Drosophila pre-mRNA splicing factor that affects the splicing of some genes in animals. A B52 deficiency in fruit flies is lethal at the larval stage, which indicates the essential and non-redundant functions of B52 in vivo. The genome-wide gene expression profile of B52 null mutant embryos using DNA microarray was previously analyzed to determine the molecular basis for the requirement B52 in normal fly development. In this study, the genes whose expression is altered by B52 were examined by analyzing the expression of several genes in two in vivo animal models, anti-B52 aptamerexpressed fly and B52 null mutant fly. The results confirmed that the Osiris genes are genuine B52-dependent genes. Overall, an inhibitory aptamer-expressed animal is a useful alternative knockout animal model for evaluating B52 loss-of-function. |
| Keyword | B52/SRp55, Gene knockout, RNA aptamer,Drosophila melanogaster |
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