| Info. | Vol.3 - No.1 (2009.03.20) |
|---|---|
| Title | Protein Interactions on Nano-Scale Controlled Surface |
| Authors | Hyo Jung Kim1, Jae-Chun Ryu2 & Soon Jin Oh1 |
| Institutions | 1Department of Biomedical Science and Technology,
Institute of Biomedical Science and Technology, Konkuk University,
Seoul 143-701, Korea 2Cellular and Molecular Toxicology Laboratory, Korea Institute of Science & Technology, P.O. Box 131, Cheongryang, Seoul 130-650, Korea Correspondence and requests for materials should be addressed to S.J. Oh (sjoh@konkuk.ac.kr) |
| Abstract | In this study, antibody microarrays on a nano-scale controlled surface were prepared. Aspects of performance such as signal intensity, dependence of signal intensity on target antigen concentration, etc. were evaluated and compared with those of microarrays on amine, aldehyde, and epoxy surfaces. The signal intensities of the anti-TNF-慣 antibody microarray fabricated on the nano-scale controlled surface were found to be 2-8 times higher than those prepared on the other surfaces. Additionally, the anti-TNF-慣 and anti-IL-1棺 antibody microarrays evidenced linear correlations between their signal intensities and concentrations of target antigen applied to the microarrays in a range between 3.0 nM-1.0 關M. Furthermore, the antibody microarrays detected two different antigens simultaneously with similar signal intensity to those achieved in single antigen etection experiments. |
| Keyword | Protein interaction, Protein microarray, Antibody, Antigen, Nano-scale controlled surface |
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