| Info. | Vol.3 - No.1 (2009.03.20) |
|---|---|
| Title | Comparison Study between Dialysis Electrode and CNT Probe for Neurotransmitter Monitoring |
| Authors | Gi Ja Lee1,2, Seok Keun Choi3, Yun Hye Eo1,2,Ji Eun Lim1,2, Jong Ho Han1,2, Samjin Choi1,2,Jeong Hoon Park1,2, Dong Hee Son4, S. Hong4, Bum Seok Oh1,2,5 & Hun Kuk Park1,2,5 |
| Institutions | 1Department of Biomedical Engineering, School of Medicine,
Kyung Hee University 2Healthcare Industry Research Institute, Kyung Hee University 3Department of Neurosurgery, Kyung Hee University Medical Center 4School of Physics and Astronomy, Seoul National University 5Program of Medical Engineering, Kyung Hee University, Seoul, Korea Correspondence and requests for materials should be addressed to H.K. Park (sigmoidus@khu.ac.kr) |
| Abstract | The performance of CNT network junction probe and conventional dialysis electrode was compared for theefficiency of real time glutamate monitoring in 11 vessel occlusion (11 VO) rat model. The dialysis electrode (Sycopel Scientific Ltd., NE 38 9BZ, U.K.) was perfused with fresh phosphate buffer saline (PBS) solution containing glutamate oxidase at 0.5 ?/min during the measurement. The glutamate oxidase immobilized CNT probe was implanted in the brain of the rat model. From the results of real time glutamate monitoring and cerebral blood flow (CBF) in 11 VO rat model, it seemed that the dynamics of glutamate release in extracellular fluid were similar both by dialysis electrode or CNT probe. However, the response of CNT probe based biosensor with L-glutamate oxidase (GLOD) to glutamate was faster (⁄10 sec) than that of dialysis electrode (?0 sec). The sensitivity of CNT probe to glutamate was higher than that of dialysis electrode in the rat model (approx. 20 times). Therefore, we suggest that the GLOD-immobilized CNT probe provides a fast response time and a high sensitivity for glutamate which are essential for the real-time glutamate monitoring. |
| Keyword | CNT probe, Dialysis electrode, Neurotransmitter, Real-time monitoring |
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