| Info. | Vol.3 - No.2 (2009.06.20) |
|---|---|
| Title | Fabrication of Electrical Cell Chip for the Detection of Anticancer Drugs and Environmental Toxicants Effect |
| Authors | Waleed Ahmed El-Said1, Cheol-Heon Yea2, Il-Keun Kwon3 & Jeong-Woo Choi1,2 |
| Institutions | 1Interdisciplinary Program of Integrated Biotechnology, Sogang University, #1 Shinsu-dong, Mapo-gu, Seoul 121-742, Korea 2Department of Chemical & Biomolecular Engineering, Sogang University, #1 Shinsu-dong, Mapo-gu, Seoul 121-742, Korea 3Department of Oral Biology, School of Dentistry, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Korea Correspondence and requests for materials should be addressed to J.-W. Choi (jwchoi@sogang.ac.kr) |
| Abstract | The effect of anticancer drugs and toxins on the viabiity of HepG2 cells were examined by the cyclic voltammetry (CV) and the potentiometric stripping analysis (PSA) methods. The cells were immobilized on gold patterned silicon substrate. The voltammetric behaviors of HepG2 cells showed a quasi-reversible process and the peak current showed a linear relationship with cell number. The attached living cells were treated with different concentrations of anticancer drugs and toxin. As the exposed concentrations of anticancer drugs and toxins were increased, we observed that the peak current in CV assay and the area under the peak in PSA assay were decreased. Trypan blue dyeing experiment was performed to confirm the results of the effects of anticancer drugs on the cell viability which were obtained from CV assay and PSA assay. These results indicate that the proposed direct cell immobilization method technique can be applied to construct the cell chip for the diagnosis, drug detection, and on-site monitoring depended on the voltammetric and PSA methods. |
| Keyword | Cell chip, HepG2 cell, Cyclic voltammetry, Potentiometric stripping analysis, Anticancer drugs effect |
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