| Info. | Vol.4 - No.3 (2010.09.20) |
|---|---|
| Title | Global transcriptome analysis of the Escherichia coli O157 response to Houttuynia Cordata Thunb |
| Authors | Ki Suk Kim1,??/sup> , You Jin Park1,??/sup> , Hee-Jae Jung1 , Kwang Seok Ahn1 , Seok-Geun Lee1 , Sung-Hoon Kim1 , Sung-Ki Jung1 & Hyeung-Jin Jang1 |
| Institutions | 1,College of Oriental Medicine, Institute of Oriental Medicine,
Kyung Hee University, 1 Heogi-dong, Dongdaemun-gu, Seoul 130-701, Korea ??/sup>These authors contributed equally to this work Correspondence and requests for materials should be addressed to S.-K. Jung and H.-J. Jang (skjang@khu.ac.kr, hjjang@khu.ac.kr) |
| Abstract | Houttuynia Cordata Thunb (HCT; Sauru-raceae), known as ?쁄osungcho??in Korea, has been traditionally used for antibiotic treatment and has proved its effect through clinical demonstrations. In spite of it s being used quite frequently in traditional prescriptions because of its superior antibiotic effect, HCT?셲 molecular mechanisms on its antibiotic effect have not been understood. Hence, this is the first study about HCT?셲 antibiotic mechanisms at the molecular level. HCT was extracted to acquire the fractions, then inoculated to confirm its antibiotic effect toward the bacteria Escherichia coli O157:H7 (E. coli O157:H7; ATCC 43894) through the isc diffusion method. The antibacterial efficacy of HCT was measured by diameter of cleared zone. Thereafter, we isolated the RNA of affected cells and analyzed it?셲 expressions through the microarray. To confirm the accuracy of the acquired data, RT-PCR was carried out. Results from this study indicate that E. coli O157:H7 exposures to HCT fractions, a number of genes that have been related to the synthesis of bacterial cell wall are downregulated, while some of the cell wall synthesis inhibitory genes are upregulated. Both results indicate that HCT fractions inhibit cell wall synthesis as like as 棺-lactam antibiotics. Furthermore, a few genes that have a critical role to DNA replication are downregulated. These genes, including folA, are closely related to the folate biosynthesis. Remarkable abnormal regulations that show antibiotic resistance activity of the E. coli O157: H7 are found, and are seem to be closely linked to the multidrug efflux pumps, especially upregulation of marRAB operon. The results of this study improve our understanding of the mode of action of HCT on E. coli O157:H7 and may show the usefulness of HCT fractions in the antibacterial treatment. |
| Keyword | Houttuynia Cordata Thunb, Antibiosis, folA, Multidrug efflux pump, Cell wall inhibition |
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