| Info. | Vol.4 - No.4 (2010.12.20) |
|---|---|
| Title | Environmental factors that affect Streptococcus mutans biofilm formation in a microfluidic device mimicking teeth |
| Authors | Wahhida Shumi1, Jeesun Lim2, Seong-Won Nam2, Kangmu Lee2, So Hyun Kim2, Mi-Hyun Kim3, Kyung-Suk Cho1 & Sungsu Park2 |
| Institutions | 1Department of Environmental Science and Engineering, Ewha Womans University, Seoul 120-750, Korea 2Department of Chemistry and Nano Science (BK21), Ewha Womans University, Seoul 120-750, Korea 3Department of Biological & Chemical Engineering, Yanbian University of Science & Technology, Yanji City 133000, China Correspondence and requests for materials should be addressed to S. Park ( nanopark@ewha.ac.kr) |
| Abstract | Streptococcus mutans is the primary etiological agent responsible for dental caries. Microfluidic devices have been used to provide a level of control over bacterial microenvironments under laminar flow conditions. In this study, we used a microfluidic device packed with glass beads to simulate the interproximal space, which is the space between the teeth. In the device, the effects of environmental factors, such as sucrose and metal ions, on S. mutans attachment and biofilm formation were quantitatively measured using confocal laser scanning microscopy and atomic force microscopy. We determined that sucrose was required for both bacterial attachment and exopolysaccharide (EPS) production in S. mutans. These results suggest that the in vivo condition between the teeth was successfully mimicked and that the device is highly suitable for in situ monitoring of oral biofilms. |
| Keyword | Streptococcus mutans, Microfluidic device, Teeth, Biofilm, Environmental factors |
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