| Info. | Vol.9 - No.3 (2015.09.20) |
|---|---|
| Title | Anti-angiogenic Function of a Korean Ginseng and Toad Venom Complex, Doksamsumsu-dan (DSSSD) Analyzed by a Forwarded Phase Antibody Microarray |
| Authors | Hwajung Kang1,??/sup>, Byung-Rok Park4,??/sup>, Hwa-Seung Yoo4,*, Ki-Rok Kwon5 & In-Cheol Kang1,2,3,* |
| Institutions | 1Department of Biological Science, College of Natural Science 2BioChip Research Center 3InnoPharmasceen Inc., Hoseo University, Asan 336-795, Korea 4East-West Cancer Center, Dunsan Oriental Medical Hospital of Daejeon University, Daejeon 302-122, Korea 5Research Center of Pharmacopuncture Medicine, Korean Pharmacopuncture Institute, Seoul 157-801, Korea |
| Abstract | We investigated anti-angiogenic effects of Korean Ginseng and Toad venom complex, Doksamsumsu-dan (DSSSD) using a Forwarded Phase Protein Array (FPPA) technology. DSSSD significantly inhibited bFGF-induced the proliferation and migration of human umbilical vein endothelial cell (HUVEC)s in a dose dependent manner. FACS analysis was carried out to confirm this inhibition by demonstrating increase of apoptosis in HUVECs treated with DSSSD. The cell apoptosis was due to stimulating Bid expression by western blotting analysis. Blood vessel formation in a chick chorioallantoic membrane (CAM) treated with DSSSD was markedly suppressed compared with a PBS-treated control group. To investigate more detail the mode-of-action mechanism of DSSSD, we used a FPPA-based antibody microarray to analyze differential expression profiling of cellular signaling proteins. We found up-regulated expression of SAM 68, a RNAbinding protein known to be involved in cell proliferation, migration, and apoptosis. These results suggest that inhibition of angiogenesis by DSSSD may be one of anti-cancer effects of DSSSD. |
| Keyword | Angiogenesis, DSSSD, Antibody microarray, HUVEC, CAM |
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