| Info. | Vol.12 - No.3 (2018.09.20) |
|---|---|
| Title | A Simple Pipetting-based Method for Encapsulating Live Cells into Multi-layered Hydrogel Droplets |
| Authors | Ju Hun Yeon1,2, Sung Hee Chung3, Changyoon Baek3, Hyundoo Hwang4 & Junhong Min3,* |
| Institutions | 1Department of Integrative Biosciences, University of Brain Education, Cheonan, Republic of Korea 2Korea Institute of Brain Science, Seoul, Republic of Korea 3School of Integrative Engineering, Chung-Ang University, Seoul, Republic of Korea 4BBB Inc., Seoul, Republic of Korea *Correspondence and requests for materials should be addressed to J. Min (junmin@cau.ac.kr) |
| Abstract | Here, we present a simple pipetting-based approach for generating multi-layered core-shell hydrogel droplets composed of alginate for outer shell and collagen for culturing cells inside. By using a multi-hole plastic substrate and by pipetting, multi-layered hydrogel droplets were generated in a simple and rapid manner. HEK293 cells, which are human embryonic kidney cells, were cultured for 14 days in double-layered hydrogel droplet with high viability. Cancer cells were co-cultured with epithelial cells in multi-layered hydrogel droplets and applied for drug tests with curcumin. As epithelial cells protect cancer cells from anti-cancer drugs, co-cultured cells showed lower sensitivity to curcumin. We developed a simple and easy method for creating complex hydrogel particles for 3D multicellular co-culture and developed an alternative method for drug testing in vivo. |
| Keyword | Multi-layered, Hydrogel, Droplets, Encapsulate, HEK293 cell, Drug assays |
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