| Info. | Vol.2 - No.2 (2008.06.20) |
|---|---|
| Title | SELEX: Combinatorial Library Methodology and Applications |
| Authors | Sung-Kun Kim1, Ben C. Shin1, Taylor A. Ott1, Wonseok Cha1, Im-Joung La2 & Moon-Young Yoon2 |
| Institutions | 1Department of Chemistry and Biochemistry and the Institute of Biomedical Studies, Baylor University, Waco, Texas 76798-7348, USA2Department of Chemistry, Hanyang University, Seoul 133-791, Korea Correspondence and requests for materials should be addressed to S.-K. Kim (sung-kun_kim@baylor.edu), M.-Y. Yoon (myyoon@hanyang.ac.kr) |
| Abstract | The SELEX (Systematic Evolution of Ligands by EXponential enrichment) technology is utilized to find aptamers, target-binding oligonucleotides, by in vitro selection. A wide variety of methodologies would be utilized to obtain the aptamers from the randomly degenerate production of oligonucleotides. An aptamer can be modified to have high specificity and affinity to their target. For example, single-stranded oligonucleotides (ssDNA or RNA) and double-stranded oligonucleotides (dsDNA) have been used to select aptamers as anti-infectional drugs or sensitive disease-detecting materials. The aptamers permit chemical structure modifications which enhance their stability and provide better applications. SELEX would be a promising future biotechnology for developing therapeutic and diagnostic agents. |
| Keyword | SELEX, Aptamer, In vitro selection, Nucleotides, Combinatorial library |
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