| Info. | Vol.3 - No.4 (2009.12.20) |
|---|---|
| Title | Capacitive Immunoaffinity Biosensor by Using Diamond-like Carbon (DLC) Electrode |
| Authors | Ju-Kyung Lee1, Gun-Ho Noh2 & Jae-Chul Pyun1 |
| Institutions | 1Department of Materials Science and Engineering, Yonsei University, 134 Sinchon-dong, Seodaemun-gu, Seoul 120-749, Korea 2Korea Institute of Science and Technology, Seoul, Korea Correspondence and requests for materials should be addressed to J.-C. Pyun (jcpyun@yonsei.ac.kr) |
| Abstract | A new capacitive biosensor based on chronoamperometry was developed by using diamond-like carbon (DLC) film as an electric isolating layer. The electrochemical properties of the DLC layered electrode were analyzed by using a cyclic voltammetry. The immunoaffinity layer was prepared by physically adsorbing the anti-horseradish peroxidase (HRP) antibodies on the DLC electrode, and the feasibility of this capacitive biosensor was tested by using HRP as a model analyte. The limit of detection (LOD) was measured to be 0.01 pg/mL, and the detection range was determined to range from less than 0.1 pg/mL to more than 1 ng/mL. The applicability of the new capacitive biosensor was demonstrated by detecting C-reactive protein (CRP) which is known as a biomarker for the inflammatory diseases. |
| Keyword | Capacitive biosensor, Diamond-like carbon (DLC), Chronoamperometry, Immunoaffinity |
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