| Info. | Vol.4 - No.1 (2010.03.20) |
|---|---|
| Title | Microchip-based capillary electrophoretic analysis of telomerase activity for cancer diagnostics |
| Authors | Se Jin Kim1, Seok Jin Choi1, Rameshkumar Neelamegam1 & Tae Seok Seo1 |
| Institutions | 1Department of Chemical and Biomolecular Engineering (BK21 Program) and Institute for the BioCentury, KAIST, Daejeon 305-701, Korea Correspondence and requests for materials should be addressed to T.S. Seo (seots@kaist.ac.kr) |
| Abstract | Here we present telomerase activity screening using a capillary electrophoretic (CE) microdevice for human cancer diagnostics. The telomerase enzyme, a contributor to the maintenance of telomere length in cancer cells, was extracted from various human cells including MCF-7 (Human breast cancer cells), A549 (Human lung cancer cells), and SK-N-SH (Human neuroblastoma cells), and then telomeric repeat amplification protocol (TRAP)-based genetic analysis was performed to evaluate the activity of telomerase enzyme. The resultant 6-bp tandem repeat PCR amplicons derived from cancer cells were separated and detected on the 6 cm-long CE microdevice within 5 min. A limit of detection of 5 cells was achieved to analyze the telomerase activity on a CE microchip. In comparison with a conventional PAGE method, microfabricated CE-based analysis has many advantages in terms of high speed, low sample consumption, and high sensitive detection. The successful demonstration of telomerase activity screening on a chip implies great potential of a complete integration of a sample preparation and PCR unit in a single format for highperformance cancer diagnostics in a clinical arena. |
| Keyword | Telomerase, Telomeric repeat amplification, Polymerase chain reaction, Capillary electrophoretic microdevice, Cancer diagnostics |
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