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Info. Vol.4 - No.4 (2010.12.20)
Title Analysis of anti-angiogenic mechanism of HangAmDan-B(HAD-B), a Korean traditional medicine, using antibody microarray chip
Authors JI-Young Bang1,??/sup>, Eung-Yoon Kim1, Tae Kyung Shim4,??/sup>, Hwa-Seung Yoo4, Yeon-Weol Lee4, Yong-Soo Kim4, Chong-Kwan Cho4, Yongjin Choi3, Hyun-Ja Jeong3 & In-Cheol Kang1,2,3
Institutions 1InnoPhamasceen Inc. and 2Department of Biological Science, College of Natural Science, 3BioChip Research Center, Hoseo University, Asan 336-795, Korea 4East-West Cancer Center, Dunsan Oriental Medical Hospital of Daejeon University, Daejeon 302-122, Korea ??/sup>These authors contributed equally to this work. Correspondence and requests for materials should be addressed to I.C. Kang and H.S. Yoo ( ickang@hoseo.edu, altyhs@hanmail.net)
Abstract The inhibitory effects of the water extract of HangAmDan-B(WEHAD-B), which is a crude extract of eight Korean medicinal animals and plants on bFGF-induced eovascularization were investigated. WEHAD-B significantly prevented bFGF- nduced HUVE cell proliferation, adhesion, migration, and capillary- like tubular etwork formation. Half-maximal inhibition of proliferation on the endothelial cells by WEHAD-B was observed at a concentration of approximately 250 關g/mL. Our tibody microarray-based ProteoChip data showed that WEHAD-B increased the xpression of STAT1 and Rb2, which are involved in cell growth, apoptosis, and controlling the cell cycle in bFGF-induced HUVECs. These results indicate that the inhibition of bFGF-induced angiogenesis by WEHAD-B may be due to upregulation of cell signaling proteins, STAT1 and Rb2. The blood vessel formation in a chick chorioallantoic membrane (CAM) treated with WEHAD-B was markedly reduced in length compared with a PBS-treated control group. Taken together, these data suggest that antibody-arrayed ProteoChip technology may be an useful tool for determining molecular mechanism of natural products in biological samples.
Keyword HAD, Anti-angiogenesis, Antibody chip array, Proteomic profiling, HUVECs
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