| Info. | Vol.5 - No.1 (2011.03.20) |
|---|---|
| Title | Passive micromixer integration with a microfluidic chip for calcium assay based on the arsenazo III method |
| Authors | Yuwadee Boonyasit1, Thitima Maturos2, Assawapong Sappat2, Apichai Jomphoak2, Adisorn Tuantranont2 & Wanida Laiwattanapaisal3 |
| Institutions | 1Graduate Program in Clinical Biochemistry and Molecular Medicine, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, 10330, Thailand 2Nanoelectronics and MEMS Laboratory, National Electronics and Computer Technology Centre, Pathum Thani, 12120, Thailand 3Centre for Excellence in the Omics-Nano Medical Technology Project Development, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, 10330, Thailand Correspondence and requests for materials should be addressed to W. Laiwattanapaisal (wanida.k@chula.ac.th) |
| Abstract | The ability for low reagent consumption and minimum waste production in a miniaturised system has generated great interest in the green chemistry field. Herein, a microfluidic system for calcium assays using the arsenazo III method has been developed. The reaction between arsenazo III and calcium to form a blue-purple coloured complex is measured by an embedded miniature fibre optic spectrometer through absorbance increments at 650 nm. A linear range was obtained from 0.2 to 3mg dL-1 with a detection limit of 0.138mg dL-1 (S/N= =3). The method exhibited good reproducibility based on low and high calcium tests with control serums, the within-run coefficient of variation (CVs) (4.10% and 3.91%), and the run-to-run CV (4.6%) were obtained. The carry-over effect of the method was also 1.98%, which is acceptable for the current system. When compared to a conventional spectrophotometric method, this portable, microfluidic method correlated highly when evaluating serum samples (r2 = =0.985; n= =15). This similarity suggests that our proposed system could be used for determining the amount of calcium in serum samples. |
| Keyword | Arsenazo III, Calcium determination, Portable microfluidic system, Passive micromixer |
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