| Info. | Vol.6 - No.3 (2012.09.20) |
|---|---|
| Title | Optimized magnetic bead-based immunoassay for automated detection of protein toxins |
| Authors | Deok-Ho Jung1, Kyoungmi Min1, Yongkwon Jeon1, Wonhee Jang2& Youngeun Kwon1 |
| Institutions | Departments of 1Biomedical Engineering and 2Life Science, Dongguk University, Pil-dong 3-ga, Seoul 100-713, Korea Correspondence and requests for materials should be addressed to Y. Kwon(ykwon@dongguk.edu) |
| Abstract | Rapid, accurate, and autonomous analysis of bioagents in the environment is critical in protecting human health from natural and intentional environmental contamination with biological toxicants. We previously developed and tested an immunoassay protocol that can be utilized for automated and simultaneous detection of selected biological agents and toxins. We adopted an antibody-based approach for the detection of pathogens and/or toxins. The fluorescent eTagsTM were used as reporter molecules and the immunoassay was modified for automated field-deployed detection of pathogens and/or toxins. The present study improved the limit of detection of this system to be suitable for the detection of environmental toxins. We tested different settings to optimize the assay protocol and successfully detected 10ng/mL(or 100fg)of an toxin analog, ovalbumin. The developed assay represents a notable improvement from currently available assays in terms of reduced time, increased sensitivity, and automation potential. Additionally, this assay can be easily modified, with the appropriate antibodies, to detect a wide range of proteins and infectious agents. |
| Keyword | Immunoassay, Magnetic bead, Autonomous detection, Toxins, Fluorescent tag |
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