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Info. Vol.8 - No.1 (2014.03.20)
Title Rapid hemagglutinin subtyping of novel avian-origin influenza A(H7N9) virus using a diagnostic microarray
Authors Dong-Hun Lee1, Ji-Hoon Kim2, Seong-Su Yuk1, Jung-Hoon Kwon1, Hyunseok Cho2, Seung Yong Hwang2,3, Hyun-Mi Kang4, Youn-Jeong Lee4, Sang-Won Lee1, In-Soo Choi1 & Chang-Seon Song1
Institutions 1Avian Diseases Laboratory, College of Veterinary Medicine, Konkuk University, Seoul, Korea
2GenoCheck Co. Ltd., Guro-gu, Seoul 152-766, Korea 3Department of Biochemistry, Hanyang University, Sangnok-gu, Ansan, Gyeonggi-do 426-791, Korea
4Avian Disease Division, Animal and Plant Quarantine Agency, 175 Anyangro, Anyangsi, Gyeonggido 430-757, Korea
Abstract An epidemic of human H7N9 influenza virus infection has recently emerged in China, caused by the novel reassortant avian-origin influenza A virus subtype H7N9. The ability to readily identify novel strains will allow a more rapid response, in order to reduce the spread of the disease and minimize the chance of a worldwide flu pandemic. We developed a low-density microarray for the rapid detection and identification of avian influenza virus subtypes H5, H7, and H9 in a previous study. In the present study, we evaluated this diagnostic microarray using an artificially synthesized novel H7N9 virus hemagglutinin gene and Korean H7 viruses isolated from wild bird habitats. Cy3-labeled DNA targets were generated by reverse transcription polymerase chain reaction using Cy3-labeled universal primers, and labeled amplicons hybridized to the microarray. All subtypes were correctly determined and identical to nucleotide sequencing results. This diagnostic microarray has enormous potential for the rapid detection of H7N9 viruses.
Keyword Influenza virus, H7N9, Diagnostic microarray, Subtyping
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