| Info. | Vol.8 - No.4 (2014.12.20) |
|---|---|
| Title | Development of Specific Immobilization Method on Gold Surface and Its Application for Determining Cardiac Risk |
| Authors | Gi Wook Kim1, Shun Zheng2, Myung Sun Kim1, Seon Ah Cheon1, Sungho Ko3 & Tae Jung Park1,* |
| Institutions | 1Department of Chemistry, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 156-756, Korea 2Department of Chemical Engineering, University of Virginia, Charlottesville, VA 22904-4741, USA 3Department of Applied Bioscience, CHA University, Seongnam, Gyeonggi-do 463-836, Korea |
| Abstract | C-reactive protein (CRP) has been regarded as a most valuable marker of inflammation that had been shown in prediction of multiple prospective blood vascular studies such as an incident myocardial infarction, stroke, peripheral arterial disease, and sudden cardiac death. A CRP detection system on a solid surface was developed using dual-antibody binding, which first antibody was immobilized by specific binding with Protein A via a fusion form with streptavidin. Second antibody bound with CRP was used for an electrochemical analysis on the gold chip surface. The fusion protein and antibodies could be successfully immobilized on the gold surface by strong biotin-streptavidin interaction as examined by surface plasmon resonance analysis. The result showed that the limit of detection has reached at 2 pg/mL. Precision is adequate, and the wide-range response was shown with the concentration of CRP between 0.01 pg/mL to 1 mg/mL. Signal difference for the same sample among different established chips is very low. Besides, the stable electrical signal was apparently maintained (~88% of initial response) for 35 days after storage at 4�C. This study provided a possibility for the development of portable chips for CRP detection, especially in the point-of-care use. |
| Keyword | Immunosensor, C-Reactive protein, Surface plasmon resonance, Electrochemical detection, Cyclic voltammetry, Amperometry |
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