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Authors
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Tae Jung Park1,2 Yun Suk Huh1, Eun Zoo Lee1, Seok Jae Lee3, Shun Zheng1, Kwang Seok Yang1, Sang Yup Lee1,2,4, Won Hi Hong1, Do Hyun Kim1 & Sungho Ko5 |
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Institutions
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1Department of Chemical and Biomolecular Engineering (BK21 program), KAIST, 335 Gwahangno, Yuseong-gu, Daejeon, 305-701, Korea 2BioProcess Engineering Research Center, Center for Ultramicrochemical Process Systems, Center for Systems and Synthetic Biotechnology, and Institute for the BioCentury, 335
Gwahangno, Yuseong-gu, KAIST, Daejeon 305-701, Korea
3NEMS-Bio Team, Department of New Technology and Analysis, National Nanofab Center, 335 Gwahangno, Yuseong-gu, Daejeon 305-806, Korea
4Department of Bio and Brain Engineering, and Bioinformatics Research Center, KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701,Korea
5Nanobiotechnology Research Group, Korea Food Research Institute, 516 Baekhyun-dong, Bundang-gu, Seongnam 463-746, Korea
Correspondence and requests for materials should be addressed
to S.Y. Lee (leesy@kaist.ac.kr), W.H. Hong (whhong@kaist.ac.kr). |
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Abstract
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A highly effective active micro mixer utilizing magnetic force with a simple onfiguration is explored to facilitate the fast mixing of more than two fluid flowsand to integrate a microfluidic system capable of disrupting cells and reacting with biomolecules. The mixing performance of the active micro mixer is evaluated within an aqueous-aqueous system including dyes for visual observation and within an organicaqueous system where enzyme reaction occurs. In addition, this magnetic stirrer for microfluidic-based research is used to create complete analytical micro systems, which facilitates cell lysis, sample preparation, and a subsequent analysis of intracellular biomolecules. Upon loading a sample of a whole cell and lysis reagent into a mixing chamber, the integrated microfluidic device carries out cell disruption by rotation of a micro magnetic-disc. Intracellular lipase samples from lyzed cells can be efficiently mixed and reacted with its substrate. We developed a micro-mixing fluidic system for cell lysis and enzyme reaction in order to detect colorimetric products. |
