| Info. | Vol.12 - No.4 (2018.12.20) |
|---|---|
| Title | Effects of Ionic Strength in the Medium on Sample Preconcentration Utilizing Nano-interstices between Self-Assembled Monolayers of Gold Nanoparticles |
| Authors | Ngoc-Viet Nguyen1, Jian-Sheng Wu1 & Chun-Ping Jen1,* |
| Institutions | Protein preconcentrator, Microfluidics, Nanochannel, Buffer solution |
| Abstract | This paper investigated the effects of ionic strength in the medium on a preconcentrator for a protein sample with low concentration. The preconcentration chip was designed and fabricated using a polydimethylsiloxane replica through standard lithophotography. A glass substrate is silanized prior to functionalizing the nanoparticles for self-assembly at a designed region. Due to the overlap of electrical double layers in a nanofluidic channel, a concentration polarization effect can be achieved using an electric field. A nonlinear electrokinetic flow is induced, resulting in the fast accumulation of proteins in front of the induced ionic depletion zone, so called exclusion-enrichment effect. Thus, the protein sample can be driven by electroosmotic flow and accumulated at a specific location. The chip is used to collect fluorescein isothiocyanate-labeled bovine serum albumin (FITC-BSA) diluted in phosphate- buffered saline (PBS) buffer solution. Different concentrations of the buffer media were studied herein. Fluorescence intensity images show that the buffer concentration of 4 mM is more appropriate than all the other ones. The sample of FITC-BSA with an initial concentration of 10 關M in the 4 mM PBS solution increases its concentration at the desired region by up to 50 times within 30 min, demonstrating the results in this investigation. |
| Keyword | 1Department of Mechanical Engineering, National Chung Cheng University, Chia-Yi, Taiwan, R.O.C. *Correspondence and requests for materials should be addressed to C.-P. Jen (imecpj@ccu.edu.tw) |
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