| Info. | Vol.13 - No.4 (2019.12.20) |
|---|---|
| Title | Fluorometric Detection of Oncogenic EML4-ALK Fusion Gene based on a Graphene Oxide System |
| Authors | Yu Mi Baek†, Yeeun Jeong† & Dong-Eun Kim* |
| Institutions | Department of Bioscience and Biotechnology, Konkuk University, Gwangjin-gu, Seoul 05029, Korea †These authors contrilbuted equally. *Correspondence and requests for materials should be addressed to D.-E. Kim (kimde@konkuk.ac.kr) |
| Abstract | Detection of the fusion gene composed of echinoderm microtubule-associated protein-like 4 (EML4) and anaplastic lymphoma kinase (ALK) is important for diagnosis of lung adenocarcinoma. We developed a simple PCR-based fluorometric method for detecting the EML4-ALK fusion using a fluorescent probe DNA and graphene oxide (GO). The fluorophore-labeled probe DNA complementary to the EML4-ALK fusion junction was hydrolyzed during PCR by the 5′ to 3′ exonuclease activity of the Taq DNA polymerase. The fluorophore released from the probe DNA retained fluorescence without adsorption onto GO. Our GO-based fluorometric system was able to detect a single cancer cell harboring the EML4-ALK fusion in 106 wild-type cells. |
| Keyword | Lung cancer cell, EML4-ALK, Fusion gene, Graphene oxide, Fluorometric detection |
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