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Info. Vol.14 - No.2 (2020.06.25)
Title Colorimetric Determination of Singlet Oxygen Scavengers Using a Protein Photosensitizer
Authors Miso Kim1, †, Eun Hye Kim1, †, Thi Hai Yen Pham2 , Tuan Anh Hoang Le2,3, Thi Phuong Do4 , Thi Nga Nguyen4 , Thi Cuc Nguyen4 , Ha Phuong Trieu4 , Thi Thao Do2,4,* & Young-Pil Kim1,5,6,*
Institutions 1 Deparment of Life Science, Hanyang University, 222, Wangsimni-ro, Seongdong-gu, Seoul 04763, Republic of Korea
2 Graduate University of Science and Technology, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Cay Giay, Ha Noi, Vietnam
3 Mientrung Institute for Scientific Research, VAST, 321 Huynh Thuc Khang Street, Thua Thien Hue, Vietnam
4 Institute of Biotechnology, VAST, 18 Hoang Quoc Viet, Cay Giay, Ha Noi, Vietnam
5 Research Institute for Natural Sciences, Hanyang University, 222, Wangsimni-ro, Seongdong-gu, Seoul 04763, Republic of Korea
6 Institute of Nano Science and Technology, Hanyang University, 222, Wangsimni-ro, Seongdong-gu, Seoul 04763, Republic of Korea
These authors contrilbuted equally.
*Correspondence and requests for materials should be addressed to Thi Thao Do (thaodo@ibt.ac.vn) and Y.-P. Kim (ypilkim@hanyang.ac.kr)
Abstract Despite the diverse roles of singlet oxygen ( 1 O2) in plants and animals, simple detection of 1 O2 has been limited due to technical difficulties. Here we report a colorimetric method for visualizing the generation and suppression of 1 O2 using a protein photosensitizer, mini singlet oxygen generator (miniSOG). Upon external light irradiation, miniSOG strongly produced 1 O2 and oxidized a universal chromogenic reagent, tetramethylbenzidine, leading to a rapid color change in solution. We used this finding to demonstrate the 1 O2 scavenging effects of well-known antioxidants and of natural compounds extracted from plants. Owing to its high usability and simplicity, our developed assay platform will be useful for screening potential scavengers for reactive oxygen species and will promote the development of various antioxidants.
Keyword Singlet oxygen, ROS, Scavenger, Colorimetry, miniSOG, Photosensitizer
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