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Info. Vol.14 - No.2 (2020.06.20)
Title Warfarin Pharmacogenetics: Single-nucleotide Polymorphism Detection using CMOS Photosensor-based Real-time PCR
Authors Seojin Bae1,2, Kukhui Son3 , Doyoung Lee2 , Sangeun Han2 , Kyunghak Choi2 & Sanghyo Kim1,*
Institutions 1 Department of Bionanotechnology, Gachon University, 1342 Seongnamdaero, Sujeong-gu, Seongnam, Gyeonggi 13120, Republic of Korea
2 OPTOLANE Inc. Pangyoyeok-ro 241, Bundang-gu, Seongnam, Gyeonggi 13494, Republic of Korea
3 Department of Thoracic and Cardiovascular Surgery, Gachon University Gil Medical Center, Gachon University, 38-13, Dokjeom-ro 3beon-gil, Namdong-gu, Incheon 21565, Republic of Korea
*Correspondence and requests for materials should be addressed to S. Kim (samkim@gachon.ac.kr)
Abstract Warfarin is a drug associated with blood clotting. As the side effects of this drug are fatal, it is important to adjust its dosage for each individual. In particular, DNA analysis of patients is necessary because the effect of the drug varies greatly depending on the genotype of the individual. Real-time PCR technology is usually used for DNA analysis. This technology requires expensive equipment, large space, and long experimental time. To solve these problems, we used a PCR module based on a CMOS photosensor. There are a heater circuit for thermal control on the its back side, and well-like structure on the surface, enabling location based multiplex PCR. We developed primers and probes to detect warfarin-related SNPs, and integrated with the PCR module. We tested the commercial Real-time PCR systems and our PCR modules. Based on the results, we confirmed that the level of performance of our system was similar to that of the commercial systems.
Keyword Real-time PCR, Warfarin, Pharmacogenetics, SNP, DNA Analysis, CMOS photosensor, PCR module
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