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Info. Vol.14 - No.4 (2020.12.20)
Title Pig Sera-derived Anti-SARS-CoV-2 Antibodies in Surface Plasmon Resonance Biosensors
Authors Ji-Hong Bong1, Tae-Hun Kim1, Jaeyong Jung1, Soo Jeong Lee1, Jeong Soo Sung1, Chang Kyu Lee1, Min-Jung Kang2, Hyun Ok Kim3 & Jae-Chul Pyun1,*
Institutions "1Department of Materials Science and Engineering, Yonsei University, 50 Yonsei-Ro, Seodaemun-Gu, Seoul 03722, Republic of Korea
2Molecular Recognition Research Center, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea
3Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul 03722, Republic of Korea
*Correspondence and requests for materials should be addressed to J.C. Pyun (jcpyun@yonsei.ac.kr) "
Abstract Anti-coronavirus disease-2019 (COVID-19; anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)) antibodies against nucleoprotein (NP) were purified from pig sera. Following the separation of the antibody fraction using a protein-A column, the final yield of the purified antibodies against SARSCoV- 2 NPs was estimated to be 0.26 ± 0.05 % (absolute amount of 143.4 ± 25.2 ng, n=5) from 1 mL of pig sera. The binding activities of the isolated antibodies were confirmed using immunoassay and immunostaining. Based on the specific binding activity to NPs, a quantitative assay was performed using a surface plasmon resonance (SPR) biosensor. From the doseresponse curve, the binding constant (Kd) was calculated to be 185 pM and the limit of detection was estimated to be 1.02 pM. The SPR biosensor with the isolated antibodies against SARS-CoV-2 NPs was applied for the detection of SARS-CoV-2, MERSCoV, and CoV strain 229E in culture fluid.
Keyword SARS-CoV-2, COVID-19, Antibody, Nucleoprotein, Surface plasmon resonance, Immunoassay
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