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Info. Vol.14 - No.4 (2020.12.20)
Title Simultaneous Multiplexed Detection of Protein and Metal Ions by a Colorimetric Microfluidic Paper-based Analytical Device
Authors Xiaolu Xiong1,2, Junlin Zhang1, Zhou Wang1, Chenchen Liu1, Wende Xiao1,2, Junfeng Han1,2,* & Qingfan Shi1,*
Institutions "1Key Laboratory of Advanced Optoelectronic Quantum Architecture and Measurement, Ministry of Education, School of Physics, Beijing Institute of Technology, Beijing, 100081, China
2Micronano Centre, Beijing Key Lab of Nanophotonics & Ultrafine Optoelectronic Systems, Beijing Institute of Technology, Beijing, 100081, China
*Correspondence and requests for materials should be addressed to Junfeng Han (pkuhjf@bit.edu.cn) and Qingfan Shi (qfshi123@bit.edu.cn) "
Abstract In order to improve the efficiency of disease diagnosis and environmental monitoring, it is desirable to detect the concentration of proteins and metal ions simultaneously, since the current popular diagnostic platform can only detect proteins or metal ions independently. In this work, we developed a colorimetric microfluidic paper-based analytical device (μPAD) for simultaneous determination of protein (bovine serum albumin, BSA) and metal ions [Fe(III) and Ni(II)]. The μPAD consisted of one central zone, ten reaction zones and ten detection zones in one device, in which reaction solutions were effectively optimized for different types of chromogenic reactions. Fe(III), Ni(II) and BSA can be easily identified by the colored products, and their concentrations are in good accordance with color depth based on the established standard curves. The detection limits are 0.1 mM for Fe(III), 0.5 mM for Ni(II) and 1μM for BSA, respectively. Best of all, we demonstrated the efficiency of the μPAD with accurate detection of Fe(III), Ni (II) and BSA from river water samples within 15 minutes. The μPAD detection is efficient, instrument-free, and easy-to-use, holding great potential for simultaneous detection of cross type analytes in numerous diagnostic fields.
Keyword Microfluidic paper-based analytical device, Colorimetric detection, Simultaneous, Fe(III), Ni(II), BSA
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