| Info. | Vol.15 - No.1 (2021.03.20) |
|---|---|
| Title | Floating Magnetic Membrane for Rapid Enrichment of Pathogenic Bacteria |
| Authors | Suhyun Kim1, Jeil Lee2, Bonhan Koo2,3, Donghoon Kwon4, Sangmin Jeon4, Yong Shin2,3(shinyongno1@yonsei.ac.kr) and Jinmyoung Joo1(jjoo@unist.ac.kr) |
| Institutions | 1Department of Biomedical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Republic of Korea 2Biomedical Engineering Research Center, Asan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea 3Department of Biotechnology, Yonsei University, Seoul 03722, Republic of Korea 4Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk 37673, Republic of Korea |
| Abstract | Efficient separation and enrichment of pathogenic bacteria from complex matrices are crucial for the detection and downstream biomedical investigations. Herein, we report a floating magnetic membrane comprised of superparamagnetic nanoparticles and cationic polymer chains for rapid capture and enrichment of pathogenic bacteria under continuous flow. Magnetic nanoparticles combined with polymeric chains have shown affordable features to capture, release, and concentrate the pathogens by applying an external magnetic field. We have verified the modulated porous characteristics of the floating magnetic membrane depending on the molecular weight of cationic polymer chains and demonstrated rapid enrichment of pathogenic bacteria from aqueous fluid in the capillary glass tube (> 50-fold). Structural flexibility of the magnetic membrane allows the liquid and smaller species to pass through but efficiently induces binding of the bacteria on the antibodyfunctionalized magnetic nanoparticles of the floating virtual web. The magnetic membrane enables size-selective filtration and target-specific trapping through ionic exchange and immunomagnetic isolation. This study implies that spatiotemporal application of the magnetic membrane for rapid enrichment of biological targets in a large volume of continuous flow using microfluidic devices and biochips. |
| Keyword | Magnetic nanoparticle · Microfluidics · Biosensor · Pathogenic bacteria · Ionic exchange |
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