| Info. | Vol.15 - No.1 (2021.03.20) |
|---|---|
| Title | Competitive Immunoassay of SARS‑CoV‑2 Using Pig Sera‑Derived Anti‑SARS‑CoV‑2 Antibodies |
| Authors | Ji‑Hong Bong1, Tae‑Hun Kim1, Jaeyong Jung1, Soo Jeong Lee1, Jeong Soo Sung1, Chang Kyu Lee1, Min‑Jung Kang2, Hyun Ok Kim3 and Jae‑Chul Pyun1(jcpyun@yonsei.ac.kr) |
| Institutions | 1Department of Materials Science and Engineering, Yonsei University, 50 Yonsei‑Ro, Seodaemun‑Gu, Seoul 03722, Republic of Korea 2Molecular Recognition Research Center, Korea Institute of Science and Technology (KIST), 5 Hwarang‑ro 14‑gil, Seongbuk‑gu, Seoul 02792, Republic of Korea 3Department of Laboratory Medicine, Yonsei University College of Medicine, 50‑1 Yonsei‑Ro, Seodaemun‑Gu, Seoul 03722, Republic of Korea |
| Abstract | Anti-severe acute respiratory syndrome coronavirus 2 (anti-SARS-CoV-2) nucleoprotein (NP) antibodies were isolated from pig sera using human SARS-CoV-2 NP-immobilized magnetic beads. The binding properties of the isolated antibodies against SARS-CoV-2 NP were tested via flow cytometry using SARS-CoV-2 NP-immobilized magnetic beads. A competitive immunoassay was developed for detecting SARS-CoV-2 NP as well as SARS-CoV-2 in the culture fluid using magnetic beads with immobilized anti-SARS-CoV-2 NP antibodies. Selectivity tests were carried out during the competitive immunoassay for SARS-CoV, MERS-CoV, and CoV strain 229E in the culture fluid. |
| Keyword | Competitive assay · SARS-CoV-2 · Culture fluid · Anti-SARS-CoV-2 nucleoprotein antibody · Nucleoprotein (NP) |
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