| Info. | Vol.15 - No.2 (2021.06.20) |
|---|---|
| Title | Development of an Inkless, Visual Volumetric Chip Operated with a Micropipette |
| Authors | Hyogu Han, Suk-Jung Choi |
| Institutions | Department of Chemistry, Gangneung-Wonju National University, Gangneung, Gangwondo 25457, Korea |
| Abstract | Visual volumetric chips based on gas-generating reactions can potentially be applied to instrument-free point-of-care testing (POCT). However, the structures of conventional chips and their detection methods are typically complicated due to two technical issues: (1) ink, as a distance marker, must be injected into a specified position before sample introduction, and (2) the gas-generating reaction must be initiated in a sealed state. In this study, an inkless volumetric chip operated with a micropipette was developed. The volumetric chip consisted of a reaction well (R well), a substrate well (S well) containing a H2O2 solution as an O2 generator, and a channel connecting the upper side of the R well and the lower side of the S well. Sample injection into the R well is followed by the insertion of a micropipette that is used to exert a negative pressure, which subsequently drives the transfer of a large portion of the H2O2 solution to the R well to initiate the gas-generating reaction. Meanwhile, a small portion of the H2O2 solution remains in the channel for use as a distance marker. The performance of the volumetric chip was evaluated by detecting Salmonella typhimurium, affording a limit of detection of 10 CFU within 20 min for culture samples and within 75 min for spiked milk samples. The volumetric chip developed herein is advantageous for POCT applications due to its simple structure and detection process. |
| Keyword | Volumetric chip · Catalase · Point-of-care testing · Pathogenic bacteria · Magnetic nanoparticles |
| PDF File |
 # 2010년도 발행분 부터는 Springer 의 BioChip Journal 페이지에서 전문을 열람하실 수 있습니다.
# 학회회원 로그인 후 [ Springer BioChip Journal 열람하기 ] 버튼을 클릭하시면 새창으로 열립니다. |
