| Info. | Vol.15 - No.3 (2021.09.20) |
|---|---|
| Title | Fully Automated System for Rapid Enrichment and Precise Detection of Enterobacteria Using Magneto-Electrochemical Impedance Measurements |
| Authors | Kirok Kwon1 · Taehee Yoon1 · Hogyeong Gwak1 · Kyungyeon Lee1 · Kyung-A. Hyun1 · Hyo-Il Jung1 |
| Institutions | *Hyo-Il Jung uridle7@yonsei.ac.kr
1School of Mechanical Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-749, Republic of Korea |
| Abstract | The contamination of food and drinking water by bacteria that cause food poisoning is a critical public health issue. In general, the procedures for testing the presence of pathogenic bacteria in public is not very effective, because it is time consuming and does not facilitate real-time monitoring in a field setting. Therefore, this study introduced a fully automated platform that would allow non-experts to easily examine the major food poisoning bacteria in a field setting, from pretreatment samples to fi nal detection. Two enterobacteria species, of four different bacteria species (i.e., Escherichia coli and Salmonella typhimurium) were successfully immobilized on the surface of magnetic beads by exploiting the specific binding force with mannose. Subsequently, magneto-electrochemical impedance measurement technology allowed bacterial detection with a high sensitivity. E. coli, typical enterobacteria, was detected in 25 min with a detection limit of 100 CFU/mL. Moreover, we demonstrated that the automatic performance and the experimental consistency was greatly improved in comparison with those of manually conducted experiments. |
| Keyword | Enterobacteria · Food poisoning · Fully automated bacterial detection · Magneto-electrochemical and mannose |
| PDF File |
 # 2010년도 발행분 부터는 Springer 의 BioChip Journal 페이지에서 전문을 열람하실 수 있습니다.
# 학회회원 로그인 후 [ Springer BioChip Journal 열람하기 ] 버튼을 클릭하시면 새창으로 열립니다. |
