| Info. | Vol.15 - No.3 (2021.09.20) |
|---|---|
| Title | A Simple Paper-Based α-Amylase Separating System for Potential Application in Biological Sciences |
| Authors | Sujin Hyung1 · Gul Karima1,2 · Kyusoon Shin1,2 · Kwang Sik Kim1,2 · Jong Wook Hong1,2,3 |
| Institutions | *Jong Wook Hong jwh@hanyang.ac.kr
1Center for Exosome and Bioparticulate Research, Hanyang University, Ansan, Gyeonggi-do 15588, Korea 2Department of Bionanotechnology, Graduate School, Hanyang University, Seoul 04763, Korea 3Department of Bionanoengineering, Hanyang University, Ansan, Gyeonggi-do 15588, Korea |
| Abstract | The salivary glands are closely associated with stress conditions and the neuroendocrine system. The concentrations of these salivary biomarkers fluctuate with a person’s disease status. Until now, salivary diagnostics have been promising; however, the analytical tools for detecting salivary biomarkers have remained burdensome due to the lack of user-friendly and costeff ective equipment. In this study, we used a microfluidic starch-coated paper-based chip to isolate α-amylase from human saliva. The concentration of α-amylase on the head and tail sections of the paper-based chip was compared in the presence or absence of starch. We found that α-amylase was highly concentrated only in the head section of the starch-coated paperbased chip through Western blotting (WB) and enzyme-linked immunosorbent assay (ELISA). We expect our designed paper-based chip will aid in α-amylase detection technologies using saliva and small amounts of other biofluids including blood, tears, pancreatic juice, and urine. |
| Keyword | Paper-based chip · Saliva · α-Amylase · Microfluidics · Biomarkers · Chronic stress · Neuroendocrine system |
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