| Info. | Vol.15 - No.3 (2021.09.20) |
|---|---|
| Title | Biomimetic Nanopillar-Based Biosensor for Label-Free Detection of Influenza A Virus |
| Authors | Wang Sik Lee1 · Junhyoung Ahn2 · Sanghee Jung3 · Jaejong Lee2,4 · Taejoon Kang 5 · Jinyoung Jeong1,6 |
| Institutions | *Jaejong Lee jjlee@kimm.re.kr
*Taejoon Kang kangtaejoon@kribb.re.kr *Jinyoung Jeong jyjeong@kribb.re.kr 1Environmental Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-Gu, Daejeon 34141, Republic of Korea 2Nano-Convergence Mechanical Systems Research Division, Korea Institute of Machinery and Materials, 156 Gajeongbuk-ro, Yuseong-gu, Daejeon 34103, Republic of Korea 3Office of Nano-Convergence Technology, National NanoFab Center, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea 4Department of Nano-Mechatronics, University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea 5Bionanotechnology Research Center, KRIBB, 125 Gwahak-ro, Yuseong-Gu, Daejeon 34141, Republic of Korea 6Department of Nanobiotechnology, KRIBB School of Biotechnology, UST, 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea |
| Abstract | Since the first emergence of influenza viruses, they have caused the flu seasonally worldwide. Precise detection of influenza viruses is required to prevent the spreading of the disease. Herein, we developed an optical biosensor using peptide-immobilized nanopillar structures for the label-free detection of influenza viruses. The spin-on-glass nanopillar structures were fabricated by nanoimprint lithography. A sialic acid-mimic peptide, which can specifically bind to hemagglutinin on the surface of the influenza virus, was immobilized onto the nanopillars via polymerized dopamine. The constructed nanopillar sensor enabled us to detect influenza A viruses in the range of 10 3 –10 5 plaque-forming units through simple measurements of reflectance. Our findings suggest that biomimetic modification of nanopillar structures can be an alternative method for the immunodiagnosis of influenza viruses. |
| Keyword | Nanopillar · Influenza virus · Peptide · Polydopamine · Reflectance |
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