| Info. | Vol.15 - No.3 (2021.09.20) |
|---|---|
| Title | On-chip Paper Electrophoresis for Ultrafast Screening of Infectious Diseases |
| Authors | Hamin Na1,2 · Byoung-Hoon Kang1,2 · Jayoung Ku2,3 · Yoosik Kim2,3 · Ki-Hun Jeong1,2 |
| Institutions | *Ki-Hun Jeong kjeong@kaist.ac.kr
1Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea 2KAIST Institute for Health Science and Technology (KIHST), Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea 3Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea |
| Abstract | The outbreak of new viral strains promotes advances in universal diagnostic techniques for detecting infectious diseases with unknown viral sequence. Long double-stranded RNA (dsRNA), a hallmark of infections, serves as a virus marker for prompt detection of viruses with unknown genomes. Here, we report on-chip paper electrophoresis for ultrafast screening of infectious diseases. Negatively charged RNAs pass through the micro and nanoscale pores of cellulose in order of size under an external electric field applied to the paper microfluidic channel. Quantitative separation of long dsRNA mimicking poly I:C was analyzed from 1.67 to 33 ng·μL −1 , which is close to the viral dsRNA concentration in infected cells. This paper-based capillary electrophoresis chip (paper CE chip) can provide a new diagnostic platform for ultrafast viral disease detection at the point-of-care (POC) level. |
| Keyword | Infectious disease · Virus detection · Long dsRNA · Paper microfluidics · Point-of-care test |
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