| Info. | Vol.16 - No.1 (2022.03.20) |
|---|---|
| Title | DNA–Gold Nanoparticle Conjugates for Intracellular miRNA Detection Using Surface-Enhanced Raman Spectroscopy |
| Authors | Won-Jun Lee1, Kyeong-Jun Kim2, Md. Khaled Hossain1, Hyeon-Yeol Cho3 & Jeong-Woo Choi1,2 |
| Institutions | * Hyeon-Yeol Cho chohy@kookmin.ac.kr * Jeong-Woo Choi jwchoi@sogang.ac.kr 1 Department of Biomedical Engineering, Sogang University, Seoul 04107, Korea 2 Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 04107, Korea 3 Department of Bio and Fermentation Convergence Technology, Kookmin University, Seoul 02707, Korea |
| Abstract | Intracellular detection of micro RNAs (miRNAs) expression is very important for cancer therapy because the expression of miRNA can regulate the chemoresistance in cancer cells. Several analysis techniques based on fluorescence have been developed for intracellular detection of miRNA, but based on those techniques it was hard to detect the low expression level of miRNA. In this study, a highly sensitive detection method of intracellular miRNA expression at the single-cell level was reported based on surface-enhanced Raman spectroscopy (SERS) using conjugated nanoparticles. Each miRNA-detectable Raman active nanoparticle (mi-RAN) consisted of gold nanoparticles (AuNP), Raman reporter, and single-stranded DNA complementary to target miRNA (miR-200c). After the internalization of mi-RANs to different subtypes of breast cancer cells, SK-BR-3 and MCF-7, SERS intensity of Raman reporter on nanoparticles were enhanced by intracellular hybridization of nanoparticles with miR-200c. According to the detection results of SERS, the expression level of miR-200c in SK-BR-3 cells was 5 times higher than that in MCF-7 cells, which was confirmed by the result of gene analysis. Moreover, treatment of mi-RAN on cancer cells was enhance chemoresistance with consumption of miR-200c. The proposed mi-RAN technique can be applied to in situ intracellular analysis platform for other miRNAs expression at the single-cell level. Moreover, mi-RAN can provide a tool to confirm the therapeutic efficacy of cancer treatment by regulating the activity of chemoresistance-related miRNA. |
| Keyword | MicroRNAs, Breast cancer cells, Intracellular detection, Surface-enhanced Raman Spectroscopy |
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