| Info. | Vol.16 - No.1 (2022.03.20) |
|---|---|
| Title | Homobifunctional Imidoester Combined Black Phosphorus Nanosheets Used as Cofactors for Nucleic Acid Extraction |
| Authors | Huifang Liu1, Qingshuang Zou1, Myoung Gyu Kim1,2, Zhen Qiao1, Dao Thi Thuy Nguyen1, Bonhan Koo1, Hyo Joo Lee1, Yoon Ok Jang1, Jun Ki Kim2& Yong Shin1 |
| Institutions | * Jun Ki Kim kim@amc.seoul.kr * Yong Shin shinyongno1@yonsei.ac.kr 1 Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, South Korea 2 Department of Convergence Medicine, Asan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, Seoul 05505, South Korea |
| Abstract | Studies on nucleic acid-based diagnostics technology have been widely applied to clinical disease diagnosis. In particular, practical effective techniques for the isolation and purification of nucleic acids from pathogenic cells are crucial to ensure the accuracy of analysis. However, numerous organic reagents from the current commercial nucleic acid extraction kits have varied potential side effects in early and further specific diagnosis. Here, we present an easy-to-operate assay for nucleic acid extraction through an outstanding two-dimensional material—black phosphorus (BP), which has attracted significant interest in the diagnosis and treatment of diseases due to its high charge carrier mobility, strong optical absorption, and excellent bioactivity. With the characterization of BP, we proposed an optimized nucleic acid extraction system. A portablesafe single 808 nm near-infrared (NIR) laser was used for the BP thermal heating system for nucleic acid extraction without any larger thermal instrument. We found that the purity and quantity of nucleic acid extracted in the BP-based system were three times higher than in a commercial kit. We also modified the nanosized BP coated with homobifunctional imidoester (HI) to enhance the biomolecule binding with both biocompatibility and surface-crosslinking abilities. We believe that the BP-HI-laser system could be a good candidate for designing nucleic acid extraction for disease diagnosis systems. |
| Keyword | Nucleic acid extraction, Black phosphorus, Homobifunctional imidoesters, Disease diagnosis system, Molecular diagnostics |
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