| Info. | Vol.16 - No.1 (2022.03.20) |
|---|---|
| Title | Thermal-Corrosion-Free Electrode-Integrated Cell Chip for Promotion of Electrically Stimulated Neurite Outgrowth |
| Authors | Seung-Min Lee1, Ji-Eun Lee1, Yun-Kyung Lee1, Da-Ae Yoo1, Da-Been Seon1, Dae-Won Lee2, Chang-Beom Kim3, Hyuk Choi4 & Kwang-Ho Lee5 |
| Institutions | * Hyuk Choi hyuk76@korea.ac.kr * Kwang-Ho Lee khmhlee@kangwon.ac.kr 1 College of Engineering, Interdisciplinary Program in Advanced Functional Materials and Devices Development, Kangwon National University, 1 Kangwondaehak-gil, Chuncheon 24341, Republic of Korea 2 Department of Chemical Engineering, College of Engineering, Kangwon National University, 1 Kangwondaehak-gil, Chuncheon 24341, Republic of Korea 3 Intelligent Robot Research Team, Electronics and Telecommunications Research Institute, Daejeon 34129, Republic of Korea 4 Department of Medical Sciences, Graduate School of Medicine, Korea University, Seoul 02841, Republic of Korea 5 Division of Mechanical and Biomedical, Mechatronics and Materials Science and Engineering, College of Engineering, Kangwon National University, 1 Kangwondaehak-gil, Chuncheon 24341, Republic of Korea |
| Abstract | In neural tissue engineering, the use of electrical stimulation has been proposed to cure patients with damaged nervous systems. Electrical stimulation can promote nerve cell differentiation and regeneration. Due to certain properties of electrode materials, including thermal problems and corrosion, the wide application of electrical stimulation has been hindered. Here, we fabricated an electro-spun, nano-porous, membrane-based, electrode-embedded cell chip capable of electrical stimulation of cells. To provide conductivity on an electro-spun nano-porous membrane, a 1.0 μm-thick layer of Au was deposited by electron beam evaporation. The electrode had a length and height of 4 and 0.025 cm, respectively, and was fixed to the inner walls of transparent polydimethylsiloxane (PDMS) cell chip channels. For 5 days, when 1 kHz of electrical stimulation was given at 50 and 100 mVpp, the promotion of neurite outgrowth of neuroblastoma SH-SY5Y cells was significantly exhibited, while no thermal problems or corrosions were detected in the electrodes. Furthermore, at 100 mVpp of electrical stimulation, not only neurite outgrowth but also secondary branch induction was observed. We believe that the newly proposed cell chip can be applied to the study of neuronal differentiation and regeneration with stable and controllable electrical stimulation. |
| Keyword | Cell chip, Corrosion-free, Electrode, Electrical stimulation, Neurite outgrowth |
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