| Info. | Vol.16 - No.2 (2022.06.20) |
|---|---|
| Title | Detection and Real-Time Monitoring of LDL-Cholesterol by Redox-Free Impedimetric Biosensors |
| Authors | Abdulaziz K. Assaifan1, Fatimah A. Alqahtani1, Sarah Alnamlah2, Rasha Almutairi1, Hend I. Alkhammash3
*Abdulaziz K. Assaifan aassaifan@ksu.edu.sa |
| Institutions | 1 King Abdullah Institute for Nanotechnology, King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia 2 College of Dentistry, King Saud University, PO Box 60169, Riyadh 11545, Saudi Arabia 3 Department of Electrical Engineering, College of Engineering, Taif University, PO Box 11099, Taif 21944, Saudi Arabia |
| Abstract | In this work, non-faradaic impedimetric biosensor was developed for the direct detection of low-density lipoprotein (LDLcholesterol). Unlike other electrochemical techniques, non-faradaic electrochemical impedance spectroscopy (EIS) does not require the use of redox probes which denatures targeted biomarkers. Furthermore, no reference electrode is required for detection which is commercially viable. The biosensor reported here consists of LDL-antibodies attached to interdigitated gold electrodes via standard chemical functionalization. The biosensor demonstrated a sensitivity and limit of detection (LoD) of 70 nF/log(ng/mL) and 120 pg/mL, respectively, where the LoD is well below the recommended LDL level in blood. The biosensor also demonstrated good selectivity toward LDL. Thereafter we utilized, for the first time, the non-faradaic impedimetric biosensor for the continuous monitoring of LDL in phosphate buffer saline (PBS). The biosensor showed rapid responses to LDL injections. Such an approach is essential for the development of low-cost and scalable wearable biosensors for the direct real-time monitoring of chronic diseases and hence allows early medical interventions in cases where sudden increases in LDL or other biomarkers are deadly. |
| Keyword | Chronic diseases · LDL detection · Non-faradaic · Real-time monitoring |
| PDF File |
 # 2010년도 발행분 부터는 Springer 의 BioChip Journal 페이지에서 전문을 열람하실 수 있습니다.
# 학회회원 로그인 후 [ Springer BioChip Journal 열람하기 ] 버튼을 클릭하시면 새창으로 열립니다. |
