| Info. | Vol.16 - No.3 (2022.09.20) |
|---|---|
| Title | One-Step Homogeneous Immunoassay for the Detection of Influenza Virus Using Switching Peptide and Graphene Quencher |
| Authors | Hong-Rae Kim1,2, Ji-Hong Bong1, Tae-Hun Kim1, Seung-Shick Shin3, Min-Jung Kang4, Won-Bo Shim5, Do Young Lee3, Dong Hee Son2, & Jae-Chul Pyun1,*
*Jae-Chul Pyun jcpyun@yonsei.ac.kr |
| Institutions | 1Department of Materials Science and Engineering, Yonsei University, 50 Yonsei-Ro, Seodaemun-gu, Seoul 03722, Korea
2Department of Chemistry, Texas A&M University, College Station, TX 77843, USA 3OPTOLANE Technologies Inc, 20 Pangyoyeok-ro 241beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do 13494, Republic of Korea 4Korea Institute of Science and Technology (KIST), 5 Hwarang-ro 14-gil, Seongbuk-gu, Seoul 02792, Republic of Korea 5Department of Agricultural Chemistry and Food Science & Technology, Gyeongsang National University, Jinju, Korea |
| Abstract | One-step homogeneous immunoassay was developed for detecting influenza viruses A and B (Inf-A and Inf-B) using the switching peptide H2. As the fluorescence-labeled switching peptide dissociated from the binding pocket of detection antibodies, the fluorescence signal could be directly generated by the binding of Inf-A and Inf-B without washing (i.e., one-step immunoassay). For the one-step homogeneous immunoassay with detection antibodies in solution, graphene was labeled with the antibodies as a fluorescence quencher. To test the feasibility of the homogeneous one-step immunoassay, the stability of the antibody complex with the switching peptide was evaluated under different pH and salt conditions. The one-step homogeneous immunoassay with switching peptide was conducted using influenza virus antigens in phosphate-buffered saline and real samples with inactivated Inf-A and Inf-B spiked in serum. Finally, the one-step homogeneous immunoassay results were compared with those of commercially available lateral flow immunoassays. |
| Keyword | One-step homogeneous immunoassay · Switching peptide · Influenza-A and influenza-B virus · Lateral flow immunoassay |
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