| Info. | Vol.17 - No.1 (2023.03.20) |
|---|---|
| Title | Development of an Integrated Biochip System Consisting of a Magnetic Particle Washing Station and a Markerless Volumetric Biochip |
| Authors | Ho Seon Choi1, Yong-Ho Jang1, Suk-Jung Choi1 *Suk-Jung Choi sjchoi@gwnu.ac.kr |
| Institutions | 1Department of Chemistry, Gangneung-Wonju National University, Gangneung, Gangwondo 25457, South Korea |
| Abstract | As the use of markers in conventional volumetric biochips results in complex biochip structures and assay processes, an integrated biochip system consisting of a magnetic particle (MP) washing station and a markerless volumetric biochip was developed in this study. The biochip was composed of a reaction chamber and a channel connected to the upper side of the chamber. The assay process involved the injection of a sample containing a catalase-labeled analyte into the reaction chamber containing a substrate solution comprising H 2 O 2, detergent, and methylene blue; and the measurement of the distance traveled by the generated foam. The MP washing station had two check valves installed at different ports of a T-connector in opposite directions. Contaminants were removed from the MPs by pressing and releasing the pipette plunger, which caused a one-way flow of washing buffer from the buffer chamber to the waste chamber through the pipette. The integrated system was applied for the detection of Salmonella typhimurium using magnetic capture particles (CPs) functionalized with anti- S. typhimurium antibodies and labeling particles (LPs) functionalized with catalase and antibodies. After the bacterial sample was mixed with CPs and LPs, the resulting CP–bacteria–LP complex was washed in the MP washing station and analyzed in the markerless volumetric biochip. This method enabled the detection of bacteria with a limit of detection of 1.6 CFU within 25 min. Considering the high sensitivity and simplicity of the biochip structure and the assay process, the integrated system demonstrates considerable potential for the point-of-care testing of various analytes. |
| Keyword | Volumetric biochip, Catalase, Point-of-care testing, Magnetic particles |
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