| Info. | Vol.17 - No.2 (2023.06.20) |
|---|---|
| Title | Development of Gut-Mucus Chip for Intestinal Absorption Study |
| Authors | Seung Yeon Lee 1, Yujeong Lee1, Nakwon Choi 2,3 , Hong Nam Kim 2,4,5,6, Bumsang Kim 1, Jong Hwan Sung 1* *Jong Hwan Sung jhsung22@hongik.ac.kr |
| Institutions | 1Department of Chemical Engineering, Hongik University, Seoul 04066 , Republic of Korea
2Brain Science Institute, Korea Institute of Science and Technology(KIST), Seoul 02792, Republic of Korea 3KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul 02841, Korea 4Division of Bio-Medical Science & Technology, KIST School, University of Science and Technology (UST),Seoul 02792, Korea 5School of Mechanical Engineering, Yonsei University, Seoul 03722, Korea 6Yonsei-KIST Convergence Research Institute, Yonsei University, Seoul 03722, Korea |
| Abstract | The intestinal epithelium is a major barrier through which orally administered drugs must pass. The intestinal mucosa on the epithelium acts as an additional barrier that protects the intestinal cells from foreign substances, thereby interfering with drug delivery. Caco-2 based cell culture model is a standard in vitro model system for testing drug uptake. However, current in vitro models do not reflect the absorption mechanism of drugs accurately due to the absence of the mucus layer. Here, we developed a microfluidic gut-mucus chip using Caco-2 cells coated with mucin protein. It was confirmed that the mucin layer was maintained under flow conditions by Alcian blue/Periodic Acid Schiff (PAS) staining. In addition, the effect of mucosal layer on drug absorption in the flow environment was examined. Mucus-adhesive particles can be useful for delivery of drugs across the intestinal epithelium. We prepared mucus-adhesive and non-adhesive microparticles containing fluorescent molecules and compared the adhesion of these particles in flow condition. Mucus-coated Caco-2 cells provide a more physiologically realistic intestinal epithelial environment to study uptake processes of drugs released from the mucus-adhesive particles. We hope that the gut-mucus chip could potentially be used as novel and more accurate in vitro models of the intestine. |
| Keyword | Intestinal mucus, Drug delivery system (DDS), Gut-on-a-chip |
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