Info.
|
Vol.17 - No.2 (2023.06.20) |
Title
|
Paper-Based Radial Flow Assay Integrated to Portable Isothermal Amplification Chip Platform for Colorimetric Detection of Target DNA |
Authors
|
Tai-Yong Kim 1,2, Sanha Kim 3, Jae Hwan Jung3, Min-Ah Woo 1
Tai-Yong Kim and Sanha Kim contributed equally to this work.
*Min-Ah Woo mawoo@kfri.re.kr |
Institutions
|
1Research Group of Food Safety and Distribution, Korea
Food Research Institution, Wanju-Gun, Jeollabuk-do 55365, Republic of Korea
2Department of Food Science and Technology, Jeonbuk
National University, Jeonju-si, Jeollabuk-do 54896,Republic of Korea
3Department of Pharmaceutical Engineering, Dankook
University, Cheonan-si,Chungcheongnam-do 31116,
Republic of Korea |
Abstract
|
A novel integrated detection system that introduces a paper-chip-based molecular detection strategy into a polydimethylsi- loxane (PDMS) microchip and temperature control system was developed for on-site colorimetric detection of DNA. For
the paper chip-based detection strategy, a padlock probe DNA (PLP)-mediated rolling circle amplification (RCA) reaction for signal amplification and a radial flow assay according to the Au-probe labeling strategy for visualization were optimized
and applied for DNA detection. In the PDMS chip, the reactions for ligation of target-dependent PLP, RCA, and labeling
were performed one-step under isothermal temperature in a single chamber, and one drop of the final reaction solution
was loaded onto the paper chip to form a radial colorimetric signal. To create an optimal analysis environment, not only
the optimization of molecular reactions for DNA detection but also the chamber shape of the PDMS chip and temperature
control system were successfully verified. Our results indicate that a detection limit of 14.7 nM of DNA was achieved, and
non-specific DNAs with a single-base mismatch at the target DNA were selectively discriminated. This integrated detection
system can be applied not only for single nucleotide polymorphism identification, but also for pathogen gene detection. The
adoption of inexpensive paper and PDMS chips allows the fabrication of cost-effective detection systems. Moreover, it is
very suitable for operation in various resource-limited locations by adopting a highly portable and user-friendly detection
method that minimizes the use of large and expensive equipment. |
Keyword
|
DNA detection, Radial flow assay, Rolling circle amplification, PDMS chip,Paper chip, Portable platform |
PDF File
|
# 2010년도 발행분 부터는 Springer 의 BioChip Journal 페이지에서 전문을 열람하실 수 있습니다.
# 학회회원 로그인 후 [ Springer BioChip Journal 열람하기 ] 버튼을 클릭하시면 새창으로 열립니다.
|