| Info. | Vol.17 - No.2 (2023.06.20) |
|---|---|
| Title | Development of an Extracellular Matrix Plate for Drug Screening Using Patient-Derived Tumor Organoids |
| Authors | Yong Hun Jung 1,6, Kyungwon Park 1, Minseop Kim 1, Hyunjik Oh 3, Dong-Hee Choi 1,6, Jinchul Ahn 1,6 , Sat-byol Lee4,5, Kyuhwan Na6, Byung Soh Min 4,5, Jin-A. Kim 1, Seok Chung 1,2
Yong Hun Jung: First author. *Byung Soh Min BSMIN@yuh.ac *Jin-A. Kim jina0160@gmail.com *Seok Chung sidchung@korea.ac.kr |
| Institutions | 1School of Mechanical Engineering, Korea University, Seoul,
Korea
2KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, Korea 3Department of Biomedical Engineering, College of Health Science, Korea University, Seoul, Korea 4Department of Surgery, Severance Hospital, Yonsei University College of Medicine, Seoul, Korea 5 Open NBI Convergence Technology Laboratory, Avison Biomedical Research Center, Yonsei University College of Medicine, Seoul,Korea 6R&D Research Center, Next&Bio Inc, Seoul, Korea |
| Abstract | With the advances in organoid culture, patient-derived organoids are utilized in diverse fields to broaden our understanding of conventional 2-dimensional (2D) culture methods and animal models. Patient-derived organoids have found new applica- tions, such as screening for patient-matching drugs, immune checkpoint drugs, and mutation-target drugs, in the field of drug screening. However, conventional dome-shaped Matrigel drop-based screening methods using 24-, 48-, and 96-well plates are not effective for carrying out large-scale drug screening using organoids. Here, we present a newly developed 96-well plate-based method for the effective screening of patient-derived tumor organoids embedded in Matrigel. The new screen- ing plate has a central hole with a diameter of 3 or 5 mm to provide a definite space for placing Matrigel in a cylindrical shape. Compared to the conventional dome-shaped Matrigel where the Matrigel drop is located arbitrarily, a cylinder-shaped Matrigel position in confined central wells allowed for faster and cost-effective tumor organoid screening. Importantly, the cylinder-shaped Matrigel ensures better consistency in high-throughput image-based analysis, which is used worldwide. Our results demonstrate the possibility of replacing the conventional 24-, 48-, and 96-well plates with the newly developed plates for effective tumor organoid screening. |
| Keyword | Cancer organoid,Drug screening, High-throughput, Organoid platform |
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