| Info. | Vol.17 - No.3 (2023.09.20) |
|---|---|
| Title | Optical-Switch Raman Spectroscopy for High Throughput Screening |
| Authors | Seung-Jin Lee 1 , Kwan Hee Lee 2, Young-Wan Choi 1, Jun Ki Kim 2,3*, Woo June Choi 1*
Seung-Jin Lee : First author. *Jun Ki Kim kim@amc.seoul.kr *Woo June Choi cecc78@cau.ac.kr |
| Institutions | 1School of Electrical and Electronics Engineering, Chung- Ang University , Seoul 06974, Republic of Korea
2Department of Medical Science, Asan Medical Institute of Convergence Science and Technology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea 3Department of Biomedical Engineering, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea |
| Abstract | Although surface-enhanced Raman spectroscopy (SERS) is a powerful analytical technique with unbeaten sensitivity, the capabilities of SERS have been not fully utilized in screening applications because throughput of spectrum detection by conventional Raman instruments has been restricted due to their single-point measurement manners. Hence, this paper presents a development of a high throughput Raman screening system that employs a fiber-optic switch and a Raman probe array. In the system, a 785 nm excitation light is directed into the 1 × 8 broadband optical switching device and selectively switched to one of 8 output ports connected to the corresponding Raman probe array to deliver the light to samples under each probe. This optical switching driven probing in sequence allows us to rapidly detect Raman scattering of the multiple (n = 8) samples in array within a short time (~ 28 s) with decent sensitivity (10 –7 M). The Raman spectroscopy of the system is validated by comparing the features of Raman spectra obtained from vitamin C tablets with those from a commercial Raman microscope and the detection sensitivity is measured with SERS substrates with different concentrations. Then, feasibility of high throughput screening is tested with a SERS chip array. |
| Keyword | Raman spectroscopy, Surface enhanced Raman spectroscopy (SERS), High throughput screening (HTS), Multi-point measurement, Optical switch |
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