| Info. | Vol.17 - No.3 (2023.09.20) |
|---|---|
| Title | Low-Powered pH-Stable Nano-electrokinetically Enhanced Lateral Flow Assay for COVID-19 Antigen Test |
| Authors | Kang Hyeon Kim 1, Yong Kyoung Yoo 2, Na Eun Lee 1,4, Junwoo Lee 1, Cheonjung Kim 2, Seungmin Lee 1,3, Jinhwan Kim 1, Seong Jun Park 1, Dongtak Lee 3, Sang Won Lee 3,5, Hyungseok Kim 1, Don Hur 1, Dae Sung Yoon 3*,Jeong Hoon Lee1*
Kang Hyeon Kim : First author. *Dae Sung Yoon dsyoon@korea.ac.kr *Jeong Hoon Lee jhlee@kw.ac.kr |
| Institutions | 1Department of Electrical Engineering, Kwangwoon University, 20 Kwangwoon-ro, Nowon, Seoul 01897, Republic of Korea
2Department of Electronic Engineering, Catholic Kwandong University, Gangneung-si, Gangwon-do 25601, Republic of Korea 3School of Biomedical Engineering, Korea University, 145 Anam-ro, Seongbuk, Seoul 02841, Republic of Korea 4 Department of Biotechnology, College of Life Sciences and Biotechnology,Korea University,Seoul 02841, Republic of Korea 5Terasaki Institute for Biomedical Innovation, Los Angeles, CA 90024 , USA |
| Abstract | Lateral flow assay (LFA) is a popular diagnostic system used in point-of-care testing (POCT) due to its low cost and portability. However, LFA has limited sensitivity and detection limits, making it challenging to detect low virus titers. Preconcentration through nano-electrokinetic (NEK) techniques have been proposed as a promising solution to improve the sensitivity of LFA. Nevertheless, the acidic conditions used in NEK operations may reduce the specificity and sensitivity of LFA immunoassays. To address these limitations, an integrated LFA kit, the NEK-enhanced LFA (PcNEK–LFA), has been introduced. This kit features a pH-controlled structure designed to facilitate sample preconcentration. Biomarkers and AuNPs are electrokinetically preconcentrated in the PcNEK–LFA platform to increase the concentration of the test line and Ag–Ab binding events, resulting in enhanced performance. The pH-controlled PcNEK–LFA platform was evaluated using salivary human chorionic gonadotropin beta (β-hCG) and COVID-19 Ag samples, achieving a preconcentrating factor of approximately 10 and a sensitivity enhancement of 55.42%, and a preconcentrating factor greater than 10, respectively. The pH-controlled PcNEK–LFA platform provides an effective solution to overcome the limitations of LFA for POCT. In addition, it improves its sensitivity and detection limit, significantly enhancing the accuracy and reliability of POCT, particularly for COVID-19 screening tests. As a result, this platform may play a pivotal role in addressing current and future healthcare challenges, facilitating rapid diagnosis and treatment of infectious diseases. |
| Keyword | Lateral flow assay, Ion concentration polarization, COVID-19 |
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