| Info. | Vol.17 - No.4 (2023.12.20) |
|---|---|
| Title | Laser-Assisted Recovery of On-Chip Phage Viral DNA for Phage Fluorescence Immunoassay Microchip |
| Authors | Seohee Chang 1 · Soohyun Kim 2 · Daewon Lee 3 · Seung Ah Lee 4 · Junho Chung 5,6,7 · Sunghoon Kwon 1,8 · Junhoi Kim7,8*
Seohee Chang: First author. *Junhoi Kim junhoi.kim@gmail.com |
| Institutions | 1Department of Electrical and Computer Engineering, Seoul National University, Seoul, Korea
2Department of Biochemistry, Stanford University School of Medicine, Stanford, CA, USA 3Department of Electronics Engineering, Myongji University, Yongin, Korea 4Department of Electrical and Electronic Engineering, Yonsei University, Seoul, Korea 5Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul National University, Seoul, Korea 6Department of Biomedical Science, Seoul National University College of Medicine, Seoul National University, Seoul, Korea 7Cancer Research Institute, Seoul National University College of Medicine, Seoul National University, Seoul, Korea 8Bio-Max Institute, Seoul National University, Seoul, Korea |
| Abstract | Laser-assisted recovery of on-chip phage viral DNA was demonstrated. Optomechanical energy conversion was utilized to shear off a part of a microchip and thereby form a micropellet. The micropellets carrying phage samples were directed to conventional laboratory circumstances for downstream analysis, addressing a ‘chip-to-world’ interface issue in lab-on-a- chip technology. Phage viral DNA content carried by the micropellets was validated through quantifi cation and sequence verification. The developed microchip configuration and its localized sample retrieval would function as a high-throughput sample handling method compatible with microchip-based phage fluorescence immunoassay. |
| Keyword | Microchip · Phage display · Immunoassay |
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