| Info. | Vol.17 - No.4 (2023.12.20) |
|---|---|
| Title | Integration of an Aptamer-Based Signal-On Probe and a Paper-Based Origami Preconcentrator for Small Molecule Biomarkers Detection |
| Authors | Na Eun Lee 1,3, Ji Hye Hong 1,2, Seungmin Lee 1,2, Yong Kyoung Yoo 5, Kang Hyeon Kim 1, Jeong Soo Park 1, Cheonjung Kim,5, Junghyo Yoon4, Dae Sung Yoon 2, Jeong Hoon Lee 1*
Na Eun Lee: First author. *Jeong Hoon Lee jhlee@kw.ac.kr |
| Institutions | 1 Department of Electrical Engineering, Kwangwoon University, Seoul 01897, Republic of Korea
2 School of Biomedical Engineering, Korea University, Seoul 02841, Republic of Korea 3 Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of Korea 4 Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, USA 5 Department of Electronic Engineering, Catholic Kwandong University, 24, Beomil-Ro 579 Beon-gilGangwon-do, Gangneung-si 25601, Republic of Korea |
| Abstract | Point-of-care testing using paper-based lateral flow assays (LFAs) has emerged as an attractive diagnostic platform. However, detecting small molecules such as cortisol using LFAs is challenging due to limited binding sites and weak signal genera- tion. Here, we report the development of cortisol-specific aptamer-based probes and a paper-based origami preconcentrator (POP) to amplify the probe signal. The cortisol-specific aptamers were conjugated onto gold nanoparticles and hybrid- ized with signal probes to create the cortisol-specific signal-on probe. POP, consisting of patterned layers with convergent wicking zones, induces electrokinetic preconcentration of the released signaling probes. By integrating cortisol-selective aptamer-based probes and POP, we accurately diagnosed cortisol levels within 30 min of signal probe incubation, followed by 10 min of preconcentration. Our sensor was able to detect cortisol levels in the range of 25–1000 ng/mL, with typical cortisol levels in plasma ranging from 40 to 250 ng/mL falling within this range. The successful detection of the wide range of cortisol samples using this approach highlights the potential of this platform as a point-of-care testing tool, particularly for lateral flow assay-based detection of small molecules like cortisol. Our approach offers a convenient and reliable method of cortisol level testing with a portable and accessible diagnosis device. |
| Keyword | Lateral flow assay · Cortisol · Paper-based origami · Preconcentration |
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